Lnc-17-92 directly interacts with c-MYC and promotes its occupancy at the ACACA promoter. (A) Western blot analysis of MYC in RPPD material precipitated with control RNA or lnc-17-92^TV1 or lnc-17-92^TV2. 5% input is used as a reference. (B) qRT-PCR analysis of lnc-17-92 (detecting lnc-17-92^TV1) in RIP material precipitated using an anti-MYC antibody (α-MYC) or immunoglobulin G (IgG) control. LncRNA PVT1 is used as a positive control for its role as MYC interactor. (C) RNA Y3H using MYC as hybrid protein 2 and, as hybrid RNAs, a negative control RNA (−) or lnc-17-92^TV1 or lnc-17-92^TV2. (D) ChIP-qPCR analysis of MYC occupancy at the ACACA promoter in AMO1, H929, and U266^MYC+ exposed for 24 hours to ASO1 or NC (vehicle). MYC occupancy at ACACA promoter is calculated as % of input chromatin. Western blot analysis of MYC from paired samples (below). GAPDH or α-tubulin were used as protein loading controls. (E) qRT-PCR analysis of ACACA mRNA in P493-6 cells exposed for 2 days to either doxycycline or DMSO to knock down MYC and then exposed for 2 additional days to either ASO1 or vehicle (NC) to deplete lnc-17-92. ACACA expression levels in cells exposed to NC were set as an internal reference. ∗P < .05, Student t test.