Skin response to DMF correlates with NF-κB, changes in microenvironment and T-cell death. (A) CODEX 7-color overlay images from skin samples of a representative responders before and after DMF study treatment. The paraffin samples were stained for CD8, CD163, CD4, β-catenin, and NF-κB p65, and the DNA dye DRAQ5 was used for nuclear staining. (B) Representative IHC pictures of skin samples from 1 responder and 2 nonresponders stained for NF-κB pp65 before and after study DMF treatment. (C) Statistical analysis of pp65 and cleaved caspase-3 staining in 3 representative responders and 3 nonresponders before and after DMF study therapy. Depicted is the percentage of positive lymphocytes from the complete lymphocytic infiltrate. ∗P < .05.