TP53 mutations are common in S0016 FL specimens and have a low VAF. (A) The pathogenic mutations detected in S0016 include canonical hotspots for TP53 mutations. The location and numbers of mutations at each site are indicated as missense (orange) or nonsense mutations (cyan). The colored regions indicate assigned functionality of each protein domain (from left, transactivation 1, transactivation 2, proline-rich domain, DNA binding, hinge domain, oligomerization domain, and alpha domain). (B) The median VAF of pathogenic mutations in FL is low. In the 37 of 147 FL specimens with a pathogenic TP53 mutation, the median VAF for these mutations is 0.02 (FL). In a validation set, 28 mutations were detected in 19 specimens (FL validation) with a median VAF of 0.03. Additional control samples of MZL and mantle cell lymphoma MCL (36 and 38 specimens) have a substantially higher median VAF. Boxes contain second and third quartiles, and whiskers 10 to 90 percentiles. Specimens with a single detectable mutation are noted with black squares. For specimens with >1 TP53 mutation, all the mutations in that specimen share a unique symbol.