Figure 1.
Effect of AZD5153 and acalabrutinib combination treatment on ABC-DLBCL. (A) TMD8 colony formation assay with methylcellulose after 14-day dosing. (Left) Representative well images for each dose with artificially labeled colonies (blue). (Right) Quantification of colonies via Alamar blue staining. Error bars the standard error of the mean (SEM) of 4 wells per cell line. (B) Cell viability scoring (0-200) across multiple doses of both drugs for 5 days in ABC-DLBCL cell lines. (C) LY0257 ABC-DLBCL PDX and (D) LY2345 GCB-DLBCL PDX models dosed with 1.5 mg/kg AZD5153 and 20 mg/kg acalabrutinib for 21 days. (E) TMD8 cell line xenograft dosed with 0.15 mg/kg AZD5153 and 20 mg/kg acalabrutinib for 26 days. (F) Body weight changes on the last day of observation. Error bars represent the SEM; n = 5 mice per group. ∗ and # denote P < .05 for comparison between the control and acalabrutinib, respectively. DMSO, dimethyl sulfoxide.

Effect of AZD5153 and acalabrutinib combination treatment on ABC-DLBCL. (A) TMD8 colony formation assay with methylcellulose after 14-day dosing. (Left) Representative well images for each dose with artificially labeled colonies (blue). (Right) Quantification of colonies via Alamar blue staining. Error bars the standard error of the mean (SEM) of 4 wells per cell line. (B) Cell viability scoring (0-200) across multiple doses of both drugs for 5 days in ABC-DLBCL cell lines. (C) LY0257 ABC-DLBCL PDX and (D) LY2345 GCB-DLBCL PDX models dosed with 1.5 mg/kg AZD5153 and 20 mg/kg acalabrutinib for 21 days. (E) TMD8 cell line xenograft dosed with 0.15 mg/kg AZD5153 and 20 mg/kg acalabrutinib for 26 days. (F) Body weight changes on the last day of observation. Error bars represent the SEM; n = 5 mice per group. ∗ and # denote P < .05 for comparison between the control and acalabrutinib, respectively. DMSO, dimethyl sulfoxide.

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