Figure 4.
AZD5153 enhances acalabrutinib-induced apoptosis. (A) Type 1 interferon response pathway protein detection of TLR4, TLR7, TRAF3, and cIAP2. (B) Alternative NF-κB pathway protein detection of p100 (including phosphorylation), p52, and IKKα. (C) Intrinsic apoptosis-related protein detection of cleaved PARP (cPARP), cleaved Cas9 (cCAS9), and Bcl-XL. (D) Superoxide detection after treatment with 20 nM AZD5153, 50 nM acalabrutinib, or a combination of both. Error bars represent SEM; n = 3. Representative histogram (middle) and pseudocolor density plots (right). (E) Cas3/7 activity normalized to CellTiter-Glo cell viability. Error bars represent SEM; n = 3. (F) Cas3/7 activity after AZD5153 treatment in cells overexpressing PAX5. Error bars represent SEM, n = 3. ∗, #, and ## denote P < .05 for comparisons of the control, acalabrutinib, and AZD5153, respectively.

AZD5153 enhances acalabrutinib-induced apoptosis. (A) Type 1 interferon response pathway protein detection of TLR4, TLR7, TRAF3, and cIAP2. (B) Alternative NF-κB pathway protein detection of p100 (including phosphorylation), p52, and IKKα. (C) Intrinsic apoptosis-related protein detection of cleaved PARP (cPARP), cleaved Cas9 (cCAS9), and Bcl-XL. (D) Superoxide detection after treatment with 20 nM AZD5153, 50 nM acalabrutinib, or a combination of both. Error bars represent SEM; n = 3. Representative histogram (middle) and pseudocolor density plots (right). (E) Cas3/7 activity normalized to CellTiter-Glo cell viability. Error bars represent SEM; n = 3. (F) Cas3/7 activity after AZD5153 treatment in cells overexpressing PAX5. Error bars represent SEM, n = 3. ∗, #, and ## denote P < .05 for comparisons of the control, acalabrutinib, and AZD5153, respectively.

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