YBX1 inhibition compromises T-ALL cell viability. (A,D) The protein expression of YBX1 was detected via western blot in Jurkat (A) and Molt4 cells (D) with YBX1 knockdown. (B,E) qRT-PCR analysis of YBX1 mRNA expression in Jurkat (B) and Molt4 cells (E). (C,F) Cell viability was determined using MTS assay in Jurkat (C) and Molt4 cells (F). (G) Western blot analysis of YBX1 in Jurkat cells with YBX1 knockout (sgYBX1-1 or sgYBX1-2). (H-I) Cell viability was assessed via MTS after transduction with the indicated lentiviruses in Jurkat cells. ∗∗P<.01; ∗∗∗P<.001.