YBX1 depletion induces apoptosis and cell cycle arrest at the G0/G1 phase in Molt4 and Jurkat cells. (A) Representative images of Wright–Giemsa staining for the morphological analysis of Jurkat and Molt4 cells. (B-C) Cell apoptosis was examined in Jurkat (B) and Molt4 cells (C) with YBX1 knockdown by flow cytometry using annexin V/PI staining. Quantification of cell apoptosis in Jurkat (B) and Molt4 cells (C). (D) Jurkat cells were infected via lentiviruses expressing YBX1 sgRNA (sgYBX1-1 or sgYBX1-2). Quantification of apoptosis in Jurkat cells with YBX1 knockout. (E) Percentages of apoptotic leukemia cells after transduction on day 3. (F) qRT-PCR analysis of mRNA expression of cell apoptosis-related genes. (G-J) Cell cycle was analyzed using flow cytometry with PI staining. (K) Relative mRNA expression of cell cycle-related genes. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.