YBX1 knockdown impedes leukemogenesis in NOTCH1-induced T-ALL model. (A) Graphical illustration of NOTCH1-induced T-ALL model. C-Kit+ stem and progenitor cells isolated from the BM of 6- or 8-week-old C57/BL6 mice were infected with a retrovirus carrying ICN1 and lentivirus expressing shC or shYBX1. Approximately 2.0 × 105 GFP+ transduced cells were intravenously injected into lethally irradiated mice. The percentage of GFP+ cells in peripheral blood was quantified using flow cytometry on the subsequent days. (B) YBX1 was analyzed before transplantation. (C) Percentages of GFP+ leukemia cells in the peripheral blood were measured via flow cytometry at 4, 5, 6, 7, and 8 weeks after transplantation. (D) Representative spleen images for each group. (E) Spleen weights of the mice in each group were measured. (F-G) GFP+ leukemia cells in the BM (F) and spleen (G) were quantified using flow cytometry on day 53. (H) Survival curves were plotted using the Kaplan-Meier curves (n = 7; long-rank test). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. The pattern diagram was drawn using Figdraw.
