Predicted target genes coregulated by NFIX and PU.1 for each hematopoietic compartment. (A) NFIX-PU.1 cobinding site prediction pipeline. The input features are normalized to ATAC-seq signals and NFIX + PU.1 motifs. The input labels are NFIX-PU.1 peaks identified from ChIP-seq in HPC5 cells. The output is cobinding probability in each 50 bp genomic bin. The tracks show high correlation between the predicted cobinding signals and observed NFIX and PU.1 signals in HPC5 on the held-out chromosome, which was chromosome 19 (see “Methods”). (B) Graph representing the total number of predicted direct target genes for NFIX-PU.1. Red bars represent the number of upregulated genes (Nfix^Δ/Δ vs Nfix^+/+) from our scRNA-seq data. Blue bars represent the number of downregulated genes from our scRNA-seq data. (C) Significant GO terms for DEG that are putatively regulated by NFIX and PU.1.