Figure 1.
Platelet thrombus formation and aggregation in KHE/KMP. Analysis of platelets from blood samples from healthy children (triangles) and patients with KHE/KMP with (circles) or without (crosses) HR. (A-B) Typical microscopy images of the growing thrombi of patients with KHE/KMP with HR (A) and healthy donors (B) after 5, 10, and 15 minutes of the whole hirudin-anticoagulated blood perfusion through fibrillar collagen-coated flow chamber. Thrombi (highlighted in red) were identified by DiOC-6 fluorescence (green). (C) Scaled thrombi areas at different time points. (D) Typical aggregation curves for the LaSca assays after activation with 800 nM ADP (black curves) or 20 μg/mL collagen (red curve). (E) Initial velocities of platelet aggregation induced by 800 nM ADP or 20 μg/mL collagen. Statistical significance was calculated using Mann-Whitney U test; ∗ P < .05.

Platelet thrombus formation and aggregation in KHE/KMP. Analysis of platelets from blood samples from healthy children (triangles) and patients with KHE/KMP with (circles) or without (crosses) HR. (A-B) Typical microscopy images of the growing thrombi of patients with KHE/KMP with HR (A) and healthy donors (B) after 5, 10, and 15 minutes of the whole hirudin-anticoagulated blood perfusion through fibrillar collagen-coated flow chamber. Thrombi (highlighted in red) were identified by DiOC-6 fluorescence (green). (C) Scaled thrombi areas at different time points. (D) Typical aggregation curves for the LaSca assays after activation with 800 nM ADP (black curves) or 20 μg/mL collagen (red curve). (E) Initial velocities of platelet aggregation induced by 800 nM ADP or 20 μg/mL collagen. Statistical significance was calculated using Mann-Whitney U test; ∗ P < .05.

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