Figure 2.
KHE/KMP affects platelet responsiveness to GPVI-mediated activation. Flow cytometry analysis of platelets and designations of groups of patients are the same as in Figure 1. (A-B) characteristics of platelets in resting state: forward light scattering (FSC) indicating platelet size (A) and cytosolic calcium concentration (B). (C-D) platelet responses to strong activation with a combination of 10 μg/mL CRP and 12.5 μM TRAP-6 (CRP + TRAP-6); or 100 μM of TRAP-6, 100 μM of AYGPKF, and 5 μM of ADP (2TR+ADP): platelet PAC-1 binding (C) and CD62p expression (D). (E-F) platelet responses to mild activation with either 2 μM of ADP (ADP), 2 μg/mL CRP (CRP) or 5 μM of TRAP-6 (TRAP-6): cytosolic calcium concentration (H) and fibrinogen binding (I). Statistical significance was calculated by means of Mann-Whitney U test. ∗P < .05; ∗∗P < .01.

KHE/KMP affects platelet responsiveness to GPVI-mediated activation. Flow cytometry analysis of platelets and designations of groups of patients are the same as in Figure 1. (A-B) characteristics of platelets in resting state: forward light scattering (FSC) indicating platelet size (A) and cytosolic calcium concentration (B). (C-D) platelet responses to strong activation with a combination of 10 μg/mL CRP and 12.5 μM TRAP-6 (CRP + TRAP-6); or 100 μM of TRAP-6, 100 μM of AYGPKF, and 5 μM of ADP (2TR+ADP): platelet PAC-1 binding (C) and CD62p expression (D). (E-F) platelet responses to mild activation with either 2 μM of ADP (ADP), 2 μg/mL CRP (CRP) or 5 μM of TRAP-6 (TRAP-6): cytosolic calcium concentration (H) and fibrinogen binding (I). Statistical significance was calculated by means of Mann-Whitney U test. ∗P < .05; ∗∗P < .01.

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