Platelet activation via CLEC-2 and GPVI receptors. Flow cytometry and low-angle light scattering aggregometry analysis and designations of groups of patients are the same as in Figure 1. (A) Initial velocity of platelet aggregation upon activation with 10 nM of rhodocytin. (B-C) Calcium mobilization (B) and fibrinogen binding (C) upon activation with 200 nM of rhodocytin. (D-G) Calcium mobilization (D,F) and fibrinogen binding (E,G) upon platelet activation with 200 nM of rhodocytin (D-E) or 2 μg/mL of CRP (F-G) for patients at the time point of enrollment (without HR, “Enroll.”) and upon HR (“HR”). Red lines correspond to patient 1, blue lines correspond to patient 9, and green lines correspond to patient 11. Green regions correspond to healthy-donor ranges. Statistical significance was calculated using Mann-Whitney U test. ∗P < .05; ∗∗P < .01.