Coronavirus infection of MO/Mφs induces expression of MLL1, coagulopathy-related factors, and inflammatory cytokines. BMDMs were harvested from C57Bl6/J mice (wild-type-BMDMs) and were infected with 1 MOI of the murine coronavirus MHVA59 for the indicated times, and mRNA (A) and protein levels (B; representative blot shown [β-actin served as loading control]) of Kmt2a/MLL1 were assayed by qRT-PCR and immunoblotting, respectively. (C) mRNA levels of factors important in CAC (Plau, Plaur, and F3) were measured in infected BMDMs. (D) mRNA levels of proinflammatory cytokines identified in the inflammatory signature resulting from acute SARS-CoV-2 (IL-6 and TNFα) and the MLL1-regulated cytokine IL-1β were measured in infected BMDMs. (E-F) Protein levels of coagulopathy-related factors (E) and proinflammatory cytokines (F) were assayed by ELISA. Bar graphs represent mean values from at least n = 5 independent experiments assayed in triplicate, and individual data points represent independent experiments. Errors bars represent SE. Statistical testing was performed using Kruskal-Wallis tests with corrections for multiple comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ELISA, enzyme-linked immunosorbent assay; MOI, multiplicity of infection; qRT, quantitative reverse transcription; SE, standard error.