MLL1 regulates the basal expression of coagulopathy-related factors and proinflammatory cytokines in BMDMs. BMDMs were harvested from mice carrying a myeloid-specific deletion of MLL1 (Kmt2a^fl/fl Lyz2Cre^+/−; denoted Cre⁺) and littermate controls (Kmt2a^fl/fl Lyz2Cre^−/−; denoted Cre−). (A) mRNA levels of Kmt2a were assayed in n = 8 Cre+ and Cre− animals analyzed in triplicate. (B) Protein levels of MLL1 were assayed in BMDMs from n = 4 Cre+ and Cre− mice by immunoblotting (representative blot shown [β-actin served as loading control]). (C-D) mRNA levels of coagulopathy-related factors (C) and proinflammatory cytokines (D) were assayed. (E-F) Protein levels of coagulopathy-related factors (E) and inflammatory cytokines (F) were measured by ELISA. (G) ChIP assays were performed using antibodies specific to either H3K4me3 or nontargeting species-specific IgG. Bars graphs represent mean values from at least n = 4 independent experiments with individual data points representing independent experiments. For ChIP experiments, bar graphs represent mean ChIP intensity relative to IgG derived from n = 8 samples performed in triplicate. Statistical analysis of pairwise comparisons was performed using Mann-Whitney tests. Error bars represent SE. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ChIP, chromatin immunoprecipitation; ELISA, enzyme-linked immunosorbent assay; IgG, immunoglobulin G; SE, standard error.