Evi1^KI/+ mice show impaired HSPCs. (A) Flow cytometric analysis of HSPCs. BMCs were obtained from the bilateral femur and tibia of Evi1^+/+ and Evi1^KI/+ age- and sex-matched littermates. The LSK compartment of Evi1^KI/+ mice was visually reduced compared with that of Evi1^+/+ mice. Representative data from 5 experiments are shown. (B) The number of LSK cells and their components. The LSK cell count of Evi1^KI/+ mice (n = 5) was significantly lower than that of Evi1^+/+ mice (n = 5). (C) Flow cytometric analysis of myeloid progenitor cells. The LK compartment of Evi1^KI/+ mice resembled that of Evi1^+/+ mice. Representative data from 5 experiments are shown. (D) The number of LK cells and its components. There was no significant difference between the number of LK cells and their components of Evi1^KI/+ mice (n = 5) and those of Evi1^+/+ mice (n = 5). Threshold significance level, P = .05 (2-tailed Welch t test). Underlined numbers denote the P value. Values are the mean ± SEM of each group. WT, Evi1^+/+ mice; KI, Evi1^KI/+ mice.