PU alters the levels of BCL-2 proteins and synergizes with VEN to induce cell death in AML independent of TP53 mutation status. (A) BCL-2 family protein levels were determined in TP53-WT and -mutant Molm13 cells treated with PU-H71. Results of a representative immunoblotting (left) and quantitative analysis of 3 independent measurements (right). (B-F) TP53-R175H Molm13 cells (B), control and MCL-1 overexpressing Molm13 cells (C), and MCL-1 knockdown (KD) by small interfering RNA TP53-WT or TP53-R175H Molm13 cells (D), (F) were treated with PU, VEN, or both for 48 hours. Cell death was determined via flow cytometry. CON, control; PU, PU-H71.