Physiologic TLR activation augments proximal BCR signaling in DLBCL. Line graphs of the normalized fluorescence intensity of pPYK2/PYK2, pSYK/SYK, and pBTK/BTK in DHL4, LY1, and LY7 cells treated with CpG (0.5, 1.25, and 2.5 μM) for 60 minutes and analyzed using in-cell western blot. Error bars represent the standard deviation of 3 independent replicates from a representative experiment. The statistical significance of the changes in fluorescence intensity at 10 and 20 minutes compared with that at baseline (time 0) was measured using the Mann-Whitney test. ∗P = .05; ∗∗P = .01.