MYD88^L265P expression increases PYK2-dependent DOCK8 phosphorylation in DLBCL. (A) Immunoblot of pTYR, DOCK8, pPYK2, and PYK2 in engineered DHL4, LY1, and LY7 cells that were untreated or treated with 50 nM PYK2 inhibitor (PF-431396) before analysis at baseline or after BCR crosslinking for 10 minutes. IgH was used as a loading control. Data are representative of 1 of 3 independent experiments. (B) Densitometry analysis of the protein bands.