Compound similarity in monoculture and coculture. (A) Joint t-SNE embedding of viable leukemia cells in monoculture and coculture controls of an AML sample. Coloring by morphological features revealed that AML cells in coculture had more elongated shapes (higher eccentricity), larger cell (Calcein), and lysosomal area as well as lower local correlation between pixel intensity values in x- and y-direction (Hoechst InfoMeas1). (B) Heatmap showing morphological changes in coculture controls across all screened disease entities. Gray indicates missing values. (C) Aggregated compound profiles were used to generate a hierarchical clustering of all probed compounds, excluding combinations. Pearson correlation was applied to compare drugs among each other separately in monoculture and coculture. Only high correlations (r > 0.4) are indicated in the heatmap. All shown correlations have P values < .001. Refer to “Materials and methods” for details.