Monocytes and T-cells do not influence CLL survival. (A) PBMCs from patients with CLL n = 5 from both groups were stained for either CD3+ or CD14+ and their representation compared. (B) CD19+ cells were isolated from CLL PBMCs from both groups, n = 3. PBMCs and CD19+ B-CLL cells were grown in RPMI for 48 hours. Cell viability was compared between PBMCs and purified CD19+ B-CLL cells. (C) Upper, hierarchical clustering of gene expression data of CMD vs CMI CLL (n = 5 each group) using Gene Cluster 3.0. Lower, principal component analysis of different CMD and CMI samples. (D) Heatmap showing differentially expressed genes between CMD vs CMI CLL (False Discovery Rate (FDR) < 0.05; Fold Change (FC) > 2). (E) Signaling pathways enriched for the differentially expressed genes in (B) using Enrichr and the Molecular Signatures Database. (F) Relative expression of genes in RAS signaling pathway and focal adhesion.