Proteomics and phospho-proteomics analysis of TRIM21 KD MM cells. (A) Quantitative proteomics analysis of MM1R-shNC and MM1R-shTRIM21 cells. We defined the differentially modifieed protein sites of shTRIM21/ shNC MM1R cells into Q1 to Q4 according to change levels. Q1: upregulated by more than twofold, Q2: upregulated by 1.5-2–fold, Q3: downregulated by 1.5-2–fold, Q4: downregulated by more than twofold. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed proteins: red indicates significant enrichment, blue indicates nonsignificant enrichment. (B) Effect of bort (5 μM; 24 hours) on the phospho-proteome profile of MM1R-shNC and MM1R-shTRIM21 cells according to a human phosphokinase array. The results are expressed as the mean pixel density. The histograms show that the 19/43 phospho-proteins are differentially expressed following bort treatment. The values are presented as mean ± SD. (∗P < .05; ∗∗P < .01; ∗∗∗P < .001).