Bmp5 deficiency causes mild iron loading in heterozygous Bmp6^het mice. Eight-week-old male (open circles) and female (closed circles) Bmp5^wt-Bmp6^wt, Bmp5^se-Bmp6^wt, Bmp5^het-Bmp6^het, and Bmp5^se-Bmp6^het mice were analyzed for liver Hamp relative to Rpl19 mRNA using qRT-PCR (A). Liver iron (B) and Tf saturation levels (C) were analyzed using colorimetric assays. (D) Liver Bmp6 relative to Rpl19 mRNA was analyzed via qRT-PCR. For panels A and D, the average of male Bmp5^wt-Bmp6^wt mice was set to 1. For all graphs, individual data points are shown, and bars represent mean ± SEM. Sex-matched data were analyzed using 1-way ANOVA with Tukey post hoc test. ∗P < .05; ∗∗ P < .01; ∗∗∗ P < .001 for the indicated comparisons.