Bmp5 deficiency exacerbates hepcidin deficiency and iron overload in endothelial Bmp6-KO mice. Eight-week-old male (open circles) and female (closed circles) Bmp5^wt-Bmp6^fl-Tek-Cre^–, Bmp5^wt-Bmp6^fl-Tek-Cre⁺, Bmp5^het-Bmp6^fl-Tek-Cre⁺-, and Bmp5^se-Bmp6^fl-Tek-Cre⁺ mice were analyzed for liver Hamp relative to Rpl19 mRNA via qRT-PCR (A). The average of male Bmp5^wt-Bmp6^fl-Tek-Cre^– mice was set to 1. Tf saturation (B), liver iron (C), pancreas iron (D), heart iron (E), and kidney iron levels (F) were analyzed using colorimetric assays. For all graphs, individual data points are shown, and bars represent mean ± SEM. Sex-matched data were analyzed using 1-way ANOVA with Tukey post hoc test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 for the indicated comparisons.