Platelet, monocyte, dendritic cell, and macrophage (myeloid) reconstitution. (A) Evolution of platelet levels after GT (left axis) and comparison to VCN in positive CFUs (left axis). (B) Comparison of mean platelet volume before and after GT (latest F/U). Statistics: Wilcoxon signed-rank test. (C) Comparison of platelet counts (≥18 months after GT) in relation to the number of transduced CD34⁺ cells infused on day 0. (D) Monocyte (CD14⁺) WASp expression (percentage WASp⁺ cells) by flow cytometry (latest F/U). (E) Evaluation of podosome formation by actin and vinculin colocalization by confocal imaging in stimulated CD14⁺ cells. (F) Evaluation of cytoskeletal function by determination of the percentage of podosome-forming cells in monocyte-derived dendritic cells (left axis) in comparison to the neutrophil VCN at 12 months (right axis). (G) Evaluation of messenger RNA expression of M1 and M2 markers by quantitative polymerase chain reaction of monocyte–derived macrophages before and after GT in patient 2 (9 months after GT) and patient 4 (3 months after GT). Graphs represent mean ± standard deviation of duplicates analyzed for each graph bar. MPV, mean platelet volume.