Figure 3.
PRMT5 inhibition promotes apoptosis through the transcription and translation of proapoptotic genes. qPCR measurement of the transcripts of select BCL-2 family genes in (A) Z-138, (B) CCMCL1, and (C) Maver-1 after 72 hours of 100 nM PRT382 treatment. Quantification of (D) BAX, (E) BAK1, and (F) BBC3 (Puma) protein levels with 6 days of PRT382 treatment. (G) Representative western blot of 6 MCL cell lines after treatment with 6 days of PRT382 or DMSO showing the levels of BAX, BAK1, and BBC3 including their cleaved forms. PRT382 doses are as follows: Z-138 150 nM, CCMCL1 100 nM, Granta 519 50 nM, Maver-1 1 μM, Mino 450 nM, and Jeko 300 nM. Note: BBC3 expression in CCMCL1 is extremely low compared with the other cell lines; an overexposed blot with CCMCL1 bands can be found in supplemental Figure 9. A student t test was used to determine significance for panels A-F. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

PRMT5 inhibition promotes apoptosis through the transcription and translation of proapoptotic genes. qPCR measurement of the transcripts of select BCL-2 family genes in (A) Z-138, (B) CCMCL1, and (C) Maver-1 after 72 hours of 100 nM PRT382 treatment. Quantification of (D) BAX, (E) BAK1, and (F) BBC3 (Puma) protein levels with 6 days of PRT382 treatment. (G) Representative western blot of 6 MCL cell lines after treatment with 6 days of PRT382 or DMSO showing the levels of BAX, BAK1, and BBC3 including their cleaved forms. PRT382 doses are as follows: Z-138 150 nM, CCMCL1 100 nM, Granta 519 50 nM, Maver-1 1 μM, Mino 450 nM, and Jeko 300 nM. Note: BBC3 expression in CCMCL1 is extremely low compared with the other cell lines; an overexposed blot with CCMCL1 bands can be found in supplemental Figure 9. A student t test was used to determine significance for panels A-F. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

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