Figure 3.
RNA-seq analysis of PIK3R1 KO cells reveals responses to Notch inhibition at transcriptional level. (A) Top significantly enriched hallmark pathways from a gene set enrichment analysis using differential gene expression results of PIK3R1 KO CB-103–treated RPMI-8402 cells compared with NT control CB-103–treated RPMI-8402 cells. (B) Heatmap showing unbiased clustering of changes in gene expression level, comparing PIK3R1 KO CB-103–treated vs NT CB-103–treated RPMI-8402 cells, highlighting key genes involved in E2F targets, PI3K-AKT-mTOR signaling, and apoptosis pathway. (C) Expression of a subset of differentially expressed genes measured as TPM comparing PIK3R1 KO CB-103–treated vs NT CB-103–treated RPMI-8402 cells. Values shown are mean ± SD. One-way ANOVA test; ∗P < .0332; ∗∗P < .0021; ∗∗∗P < .0002. TPM, transcripts per million.

RNA-seq analysis of PIK3R1 KO cells reveals responses to Notch inhibition at transcriptional level. (A) Top significantly enriched hallmark pathways from a gene set enrichment analysis using differential gene expression results of PIK3R1 KO CB-103–treated RPMI-8402 cells compared with NT control CB-103–treated RPMI-8402 cells. (B) Heatmap showing unbiased clustering of changes in gene expression level, comparing PIK3R1 KO CB-103–treated vs NT CB-103–treated RPMI-8402 cells, highlighting key genes involved in E2F targets, PI3K-AKT-mTOR signaling, and apoptosis pathway. (C) Expression of a subset of differentially expressed genes measured as TPM comparing PIK3R1 KO CB-103–treated vs NT CB-103–treated RPMI-8402 cells. Values shown are mean ± SD. One-way ANOVA test; ∗P < .0332; ∗∗P < .0021; ∗∗∗P < .0002. TPM, transcripts per million.

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