Figure 3.
Compression and squeezing of mitochondria during platelet activation observed by live cell video microscopy and FIB-SEM images. (A) Live cell video microscopy of microtubule-tracker (green) and mitoTracker (magenta) stained human platelets during activation with thrombin (final concentration 0.034 U/mL). Still images of the supplemental Video 1 are shown at different time points as indicated. White arrows indicate mitochondria that get squeezed through the coiled marginal band; scale bar, 2 μm. (B-D) Single FIB-SEM image planes of platelets after activation with 1.25 μg/mL collagen (top) and below 3D reconstructions of FIB-SEM image stacks of platelets at activation states similar to those in the top panels. The white arrow indicates a mitochondrion that gets squeezed through the encircled center; scale bars, 1 μm.

Compression and squeezing of mitochondria during platelet activation observed by live cell video microscopy and FIB-SEM images. (A) Live cell video microscopy of microtubule-tracker (green) and mitoTracker (magenta) stained human platelets during activation with thrombin (final concentration 0.034 U/mL). Still images of the supplemental Video 1 are shown at different time points as indicated. White arrows indicate mitochondria that get squeezed through the coiled marginal band; scale bar, 2 μm. (B-D) Single FIB-SEM image planes of platelets after activation with 1.25 μg/mL collagen (top) and below 3D reconstructions of FIB-SEM image stacks of platelets at activation states similar to those in the top panels. The white arrow indicates a mitochondrion that gets squeezed through the encircled center; scale bars, 1 μm.

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