Drp1-deficient platelets show a delay in clot retraction. (A) Washed platelets from WT and Drp1-KO mice were resuspended in Tyrode buffer at 3×10⁸/mL. Fibrinogen (800 μg/mL) and CaCl₂ (12.5 mM) were added, and clot formation was initiated by adding thrombin (1.7 U/mL). The clots were allowed to retract, and images were taken at indicated time points. (B) The extent of clot retraction was quantified as percent of the initial clot volume using the images shown in panel B. Results are expressed as means ± standard deviations (P = .03078 at 35 minutes). Shown is a representative experiment repeated 3 times.