Single-cell transcriptomic analysis reveals development of multiple hematopoietic cell types in an in vitro model of human hematopoietic differentiation. (A) Experimental design: in vitro culture system of hematopoiesis from human cord blood HSPCs and schema of sample processing and data analysis. (B) Different cell types were identified based on expression of a suite of lineage-specific genes (refer to “Methods”). (C) Representation using UMAP of all cells from control group collected over the 28-day period. (D) Development of cell clusters over time is shown. (E) Proportion of cells of each cluster across time. (F) Percent of cells belonging to major cell types that develop from untreated HSPCs were identified using flow cytometry. Error bars show mean ± standard error of the mean from 2 independent experiments. HSC, hematopoietic stem cells; MPP, multipotent progenitors; cDC2, type 2 classical dendritic cells; pDC, plasmacytoid dendritic cells; Meg-Ery, megakaryocyte-erythroid progenitors.