AHR activation by TCDD alters the development of several hematopoietic lineages. (A) Changes in cell clusters from vehicle and TCDD groups across the 28-day developmental period. Some of these changes are highlighted with red circles. (B) Percent of cells belonging to each scRNA-seq data associated cell cluster for Vehicle and TCDD-treated groups across the 28-day developmental period. (C) Representative UMAP plots of cells captured by flow cytometry on day 21, with major cell populations identified through expression of different lineage markers. Comparison of UMAP plots of an equal number of cells (30,000) from both vehicle and TCDD-treated groups; (D) Percent of cells belonging to major hematopoietic lineages captured using flow cytometry for vehicle and TCDD-treated groups across the 28-day developmental period. Data presented are composite of 6 independent experiments. Statistical significance of differences in percentage of cells between treatments at any time point was calculated using a 2-tailed paired t test. ∗P < .05; ∗∗P < .01; ∗∗∗∗P < .0001. Error bars show mean ± standard error of the mean. Refer to supplemental Figures 5 and 6 for gating strategy. cDC2, type 2 classical dendritic cells; CLP, common lymphoid progenitor.