Allele-selective inhibition of Vwf in vivo in B6 and 129S mice treated with siVwf.B6 or siVwf.129S. (A) Lung mRNA expression and plasma levels of VWF in B6 (blue) and 129S (red) mice treated with siVwf.B6. Plasma and lungs were collected 96 hours after injection with siVwf.B6. (B) Lung mRNA expression and plasma levels of VWF in B6 (blue) and 129S (red) mice treated with siVwf.129S. Plasma and lungs were collected 96 hours postinjection with siVwf.129S. (C) Lung mRNA expression and plasma levels of VWF of siVwf.B6-treated B6 (blue) mice and siVwf.129S-treated 129S (red) mice. Mice were sacrificed 10 days after receiving their respective siRNA treatments to indicate the effect of a single siRNA injection after a prolonged time period. n = 6 mice per group, however, incidentally a blood sample was removed because of unwanted clotting activation (as established visually). Values are presented as median with range and is compared with the median of the corresponding scrambled siControl.B6 or siControl.129S-treated mice. P ≥ 0.05, ns; ∗P ≤ 0.05; ∗∗P ≤ 0.01.