Secondary effects of allele–selective Vwf silencing. (A) The effect on plasma FVIII and platelet (Plt) VWF was assessed in plasma of siVwf.B6-treated B6 mice, and F8 transcript levels were measured on mRNA isolated from lungs of siVwf.B6-treated B6 mice. (B) The effect on plasma FVIII and Plt VWF was assessed in plasma of siVwf.B6-treated 129S mice, and F8 transcript levels were measured on mRNA isolated from lungs of siVwf.B6-treated 129S mice. The effect of plasma VWF, plasma FVIII, and F8 lung mRNA was all relative to the median of their respective siControl.B6 values (left y-axis), whereas for Plt VWF data were normalized as mU per 109 platelets (right y-axis). Individual values are presented with median. P ≥ 0.05, ns; ∗∗P ≤ 0.01. Note: plasma VWF data are the same as shown in Figure 4A and were used as reference for the other parameters of interest for clarity.
