Distinct activities of transcriptional networks/circuits revealed throughout anemia recovery timeline. (A) UMAP representation of scRNA-seq data generated from Kit⁺ spleen cells isolated from control mice and mice post-PHZ days 1, 3, and 7. Annotation indicates 21 distinct color-coded clusters. (B) Bar graph depicting HSPCs and erythroid-committed percentages relative to total Kit⁺ cells. (C) Venn diagram indicating the unique and shared upregulated genes in anemia vs control mice at indicated time points. (D) Clustered average expression over 4 time points of all E3-expressed genes. Each of 9 transcriptional states were determined by TCseq (z score represents relative vs average change in transcription level). Color bar of membership values indicate the degree to which data points conform to a transcriptional state. Number of transcripts conforming to each transcriptional state were shown in parentheses. (E) Heatmap of normalized expression level of 65 GATA/TAL1 occupied EGA genes in cells at each time points. Each column represents a single cell.