Figure 3.
Binding of VWF with varying multimer size to KB-VWF-D3.1. (A) pdVWF concentrates were applied to gel-filtration chromatography using Bio-Gel-A-15m. Fractions enriched in HMW-multimers (HMW-VWF) and medium–molecular weight multimers (MMW-VWF) were analyzed for their multimeric pattern using SDS-agarose (2%) electrophoresis. (B) Binding of HMW-VWF (purple circles) and MMW-VWF (blue circles) to immobilized KB-VWF-D3.1 (5 μg/mL). (C) Binding of multimeric rVWF (orange circles) and the dimeric VWF/delta-pro variant (green squares) to immobilized KB-VWF-D3.1 (5 μg/mL). In both panels, bound VWF was probed using peroxidase-labeled polyclonal anti-VWF antibodies and detected via hydrolysis of 3,3',5,5'-tetramethylbenzidine. Data represent mean ± SD of 3 to 4 independent measurements. OD, optical density.

Binding of VWF with varying multimer size to KB-VWF-D3.1. (A) pdVWF concentrates were applied to gel-filtration chromatography using Bio-Gel-A-15m. Fractions enriched in HMW-multimers (HMW-VWF) and medium–molecular weight multimers (MMW-VWF) were analyzed for their multimeric pattern using SDS-agarose (2%) electrophoresis. (B) Binding of HMW-VWF (purple circles) and MMW-VWF (blue circles) to immobilized KB-VWF-D3.1 (5 μg/mL). (C) Binding of multimeric rVWF (orange circles) and the dimeric VWF/delta-pro variant (green squares) to immobilized KB-VWF-D3.1 (5 μg/mL). In both panels, bound VWF was probed using peroxidase-labeled polyclonal anti-VWF antibodies and detected via hydrolysis of 3,3',5,5'-tetramethylbenzidine. Data represent mean ± SD of 3 to 4 independent measurements. OD, optical density.

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