Figure 1.
Effect of mutations on cytokine-induced STAT3 Y705 phosphorylation. (A) Schematic diagram of STAT3 depicting each domain: NTD, CCD, DBD, LD, SH2D, TAD, and NRD. (B) Representative immunoblot analysis of lysates of STAT3−/− MEF cells reconstituted with WT or mutant STAT3 constructs and incubated without (top) or with (bottom) IL6/sIL6R (100 ng/mL). Blots were probed with antibody against total (t) STAT3 or pY-STAT, as indicated. The red asterisk (∗) indicates that the images are a composite of 2 separate blots imaged simultaneously with equal exposure. (C) Bar graph showing the densitometry signal for pY-STAT3 normalized for total (t) STAT3 and the mean ± SEM of 3 immunoblots shown (∗P < .05; Student t test). CCD, coil-coiled domain; LD, linker domain; NRD, nuclear retention domain; NTD, N-terminal domain; SEM, standard error of the mean; TAD, transactivation domain.

Effect of mutations on cytokine-induced STAT3 Y705 phosphorylation. (A) Schematic diagram of STAT3 depicting each domain: NTD, CCD, DBD, LD, SH2D, TAD, and NRD. (B) Representative immunoblot analysis of lysates of STAT3−/− MEF cells reconstituted with WT or mutant STAT3 constructs and incubated without (top) or with (bottom) IL6/sIL6R (100 ng/mL). Blots were probed with antibody against total (t) STAT3 or pY-STAT, as indicated. The red asterisk (∗) indicates that the images are a composite of 2 separate blots imaged simultaneously with equal exposure. (C) Bar graph showing the densitometry signal for pY-STAT3 normalized for total (t) STAT3 and the mean ± SEM of 3 immunoblots shown (∗P < .05; Student t test). CCD, coil-coiled domain; LD, linker domain; NRD, nuclear retention domain; NTD, N-terminal domain; SEM, standard error of the mean; TAD, transactivation domain.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal