Figure 5.
Hemostatic response after mechanical injury to the large vessels. (A-F) HB and WT mice infused with PBS served as the controls. Tail clip (A) or TVT assay (C) were performed in HB mice injected with FIX-WT or FIX-V16L at the indicated doses, and blood loss (μL) was collected for 10 minutes or 60 minutes, respectively. Dose-dependent bleeding reduction with FIX-V16L or FIX-WT was determined by fitting tail clip (B) and TVT (D) data to a logistic function (solid lines). The dotted line indicates the median value for the WT mice. Data are presented as the mean ± SD from n = 7 per group. Adjusted P values were obtained using an ANOVA test followed by a Tukey test. ∗P < .05, ∗∗P < .004, or n.s. represent FIX-V16L vs FIX-WT infused animals. Bleeding profile after TVT in HB mice injected with 75 μg/kg (E) or 50 μg/kg (F) FIX protein as a function of time. Each individual point represents the average blood loss (μL) between clot disruptions from 7 mice.

Hemostatic response after mechanical injury to the large vessels. (A-F) HB and WT mice infused with PBS served as the controls. Tail clip (A) or TVT assay (C) were performed in HB mice injected with FIX-WT or FIX-V16L at the indicated doses, and blood loss (μL) was collected for 10 minutes or 60 minutes, respectively. Dose-dependent bleeding reduction with FIX-V16L or FIX-WT was determined by fitting tail clip (B) and TVT (D) data to a logistic function (solid lines). The dotted line indicates the median value for the WT mice. Data are presented as the mean ± SD from n = 7 per group. Adjusted P values were obtained using an ANOVA test followed by a Tukey test. ∗P < .05, ∗∗P < .004, or n.s. represent FIX-V16L vs FIX-WT infused animals. Bleeding profile after TVT in HB mice injected with 75 μg/kg (E) or 50 μg/kg (F) FIX protein as a function of time. Each individual point represents the average blood loss (μL) between clot disruptions from 7 mice.

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