Hemostatic response after laser-induced injury to arterioles. (A) Images depict representative thrombi induced at 1, 10, 20, 30, 40, and 60 minutes after injection of 25 μg/kg FIX-WT or FIX-V16L into HB mice. Platelets (red) were detected using an Alexa₅₅₅-labeled rat anti-CD41 F_(ab)2 and fibrin (green) Alexa₆₄₇-labeled anti-fibrin antibodies; areas of overlap are yellow. (B-C) Graphs showing the median area under the curve (AUC) for CD41 or fibrin vs time curve in HB mice injected with FIX-WT or FIX-V16L (25 μg/kg), or PBS. Each time point represents the median ± SD of 4 to 5 thrombi from 4 to 5 mice.