Genetic dependency on BCL2L1 in erythroid and megakaryoblastic leukemias. (A) Volcano plot of the most essential genes in erythroid compared with other AML cell lines in genome-scale CRISPR-Cas9 screening data from DepMap. Genes with a nominal P value < .05 and absolute values of differential gene effect >0.6 are colored in red or blue. In addition, the BCL-2 family members BCL2 and MCL1 are colored in blue. Fifteen percent FDR is indicated with a dashed line. (B) BCL2L1 CRISPR gene knockout effect in AML cell lines. More negative values indicate stronger dependency. (C) Volcano plot of most essential genes in erythroid/megakaryoblastic AML cell lines compared with other AML cell lines in RNAi screening data from the Achilles project. Genes with a nominal P value < .05 and absolute values of differential gene effect >0.5 are colored in red or blue. In addition, the BCL-2 family members BCL2 and MCL1 are colored in blue. Fifteen percent FDR is indicated with a dashed line. (D) BCL2L1 RNAi dependency score in AML cell lines. More negative values indicate stronger dependency. FDR, false discovery rate.