BCL-2 family gene expression in blasts across AML types at single-cell resolution. (A) UMAP plot of scRNA-seq data of blasts from AML with megakaryoblastic differentiation (AML-1), AML with erythroid differentiation (AML-5), and 3 AMLs representing other subtypes. Cell types identified using the reference-based method SingleR are colored. (B) UMAP plots as in panel A with patients and expression of BCL2L1, BCL2, and MCL1 colored as normalized and scaled log-transformed counts. (C) Dot plot of RNA expression of selected megakaryocyte and erythroid markers, TFs regulating erythroid/megakaryocytic differentiation, progenitor markers, and BCL-2 family genes in the indicated cell types based on reference-based method SingleR annotations. (D) Violin plot demonstrating BCL-2 family gene expression of the blasts in different patients.