Sustained long-term and multilineage output potential of CD49f⁺cells from birth to late adulthood in clonal in vitro assays. (A) Design of the stromal cell coculture system used to measure the 8-week clonal kinetics of NM cell production from individual human CD49f⁺ cells obtained from differently aged donors. (B) Proportion of individually assessed CD49f⁺ cells that showed persistent outputs to week 8 based on analyses of 898 cells from 5 CB samples, 538 BM cells from 3 young adult donors, and 539 BM cells from 3 samples of aged adult donors. (C) Distribution of clonal outputs from each donor age group (clone categories: H, highly proliferative; P, proliferative; T, transient; N, negative; ∗∗∗P < .001; Fisher exact test). (D) Cumulative distribution of the time at which each persistent clone first appeared (∗∗∗P < .001; Komoglerov-Smirnov test). (E) Design of the stromal coculture system used to measure the 6-week multilineage (lymphoid and/or NM) cell output potential of individual CD49f⁺ cells obtained from differently aged donors. (F) Representative gating strategy of indicated lineages present in the 6-week harvests of the multilineage cocultures described in panel E. (G) Average distribution of the 6-week clone types obtained from the different sources of CD49f⁺ cells tested, based on analyses of 239 cells from 2 CB samples, 240 cells from 2 young adult BM samples, and 240 cells from 2 aged adult BM samples; ∗∗∗P < .001; Fisher exact test.