Poor growth and iron depletion in IECs of PCBP1^ΔIEC mice. (A) Body weights of WT and PCBP1^ΔIEC mice aged 2 and 6 months. Weanling mice fed a tamoxifen diet for 1 month followed by a standard chow diet. (B) Reduced nonheme iron levels in the IECs of PCBP1^ΔIEC mice. IECs from 2-month-old mice were collected and analyzed for nonheme iron. (C) Lower body weights of PCBP1^ΔIEC mice on defined-iron diets. WT and PCBP1^ΔIEC mice were fed 5 ppm, 50 ppm, or 1000 ppm diets for 1 month. Body weight measured at the age 3 months. (D) Reduced nonheme iron levels in the IECs of PCBP1^ΔIEC mice on defined-iron diets. IECs from mice in panel C were analyzed for nonheme iron. (E) Lower ferritin and higher NCOA4 levels in IECs of PCBP1^ΔIEC vs WT mice. Mice were fed defined-iron diets as in panel C. IECs were collected at 3 months and analyzed by immunoblot for ferritin and NCOA4. A representative blot is shown above with quantitation below. Analyzed mice were male littermates; Female littermates were included as indicated. Refer to data from female mice in supplemental Figure 2.