Single-cell transcriptomic analysis reveals altered mitochondrial and protein translation signatures in murine HSPCs. (A) Timeline of GW4869 (or DMSO) treatment in ex vivo cultured murine HSPCs (n = 3 biologic replicates per treatment/condition) before 10x Genomics single-cell RNA sequencing. (B) Proportions of mitochondrial over total genes sequenced per cell across treatment groups and time points. (C) In silico fluorescence-activated cell sorter–like annotations of stem and progenitor cell populations identified across conditions in single-cell RNA sequencing analyses. (D) Uniform manifold approximation and projections of ST-HSCs in HSPC^Con and HSPC^GW at 24-hour time point. Bubble heatmaps of significant hallmark gene sets enriched in HSPC^GW at 24 hours (E) and 120 hours (F). (G) Violin plots of top differentially expressed genes in ST-HSC^GW. Refer to supplemental Figures 3 and 4.