Changes in TG in blood from patients with altered RBC traits. Blood was investigated from day-control subjects (Con), or from patients with polycythemia vera, erythrocytosis, or anemia of various causes (Table 1). WB TG was measured in response to vehicle, TF (0.1, 1.0 pM), or Rvv-X (dilutions 1e-5, 1e-6, 1e-7). Where indicated, platelets in blood were stimulated with CRP-XL (25 μg/mL, 0.1 pM TF). Parameters of TG curves were univariate scaled from 0 to 10 across all conditions, and differences per patient vs means of control participants were obtained. (A) Subtraction heat map of TG parameters (scaled 0-10) from 15 patient samples per indicated trigger. Note reversed scaling for lagtime and time-to-peak (TTP). Differences were filtered for changes outside of means ± SD. Color codes: blue, decrease; red, increase. (B) Plot of RBC count and thrombin peak level (0.1 pM TF) per subject. (C) Results of principal component analysis of combined TG and hematologic parameters (supplemental Figures 7 and 8). Colors indicate 4 clusters, with each dot representing 1 subject. (D-E) Effect of annexin A5 addition (vehicle, 1 or 2 μg/mL) on lagtime (D) and peak level (E) at 0.1 pM TF per cluster of subjects (clustered according to panel C). For full data, see Datafile 1. Ane, anemia; Ery, erythrocytosis; Pcv, polycythemia vera.