Figure 2.
Loss of ARID1B affects reconstitution of myeloid cells in competitive repopulation assays. (A) Frequencies of LT-HSC (CD34−Flt3− LSK), short-term (ST)-HSC (CD34+Flt3− LSK), and MPP (CD34+Flt3+ LSK) populations observed in the BM of Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice, either 4 or 16 to 20 weeks after poly(I:C) injection; 4 weeks: n = 4 for control, and n = 3 for KO mice; 16 to 20 weeks: n = 5 for control, and n = 4 for KO mice. (B) Percentages of myeloid precursors, common myeloid progenitors (CMPs), granulocyte monocyte progenitors (GMPs), and megakaryocyte erythrocyte progenitors (MEPs), in the BM of Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice after poly(I:C) injection; 4 weeks: n = 4 for control, and n = 2 for KO mice; 16 to 20 weeks: n = 6 for control, and n = 5 for KO mice. (C) Total thymocyte count in ARID1B-deficent or control mice at either 4 or 16 to 20 weeks after poly(I:C)-mediated deletion of Arid1b. (D) Proportion of double-positive (DP), double-negative (DN), CD4+ single-positive (SP), and CD8+ SP populations in Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice, either 4 or 16 to 20 weeks after poly(I:C) injection; 4 weeks: n = 7 for control, and n = 5 for KO mice; 16 to 20 weeks: n = 8 for control, and n = 7 for KO mice. (E) Donor chimerism (CD45.2+) in the peripheral blood of recipient mice in competitive repopulation assays. Lethally irradiated recipients received transplantation with either Arid1bfl/fl;Mx1-Cre+ or Arid1bfl/fl;Mx1-Cre− BM mixed in equal proportion with competitor BM (CD45.1+) and deletion of Arid1b was induced 4 weeks after transplantation using poly(I:C). Proportion of donor-derived B cells, T cells, granulocytes, and monocytes were determined every 4 weeks in the peripheral blood of recipient mice (n = 7 for Arid1bfl/fl;Mx1-Cre− and n = 8 for Arid1bfl/fl;Mx1-Cre+). Data are represented as mean ± standard error of the mean (SEM). Statistical difference between WT and KO mice was calculated using unpaired t test and is denoted as ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001.

Loss of ARID1B affects reconstitution of myeloid cells in competitive repopulation assays. (A) Frequencies of LT-HSC (CD34−Flt3− LSK), short-term (ST)-HSC (CD34+Flt3− LSK), and MPP (CD34+Flt3+ LSK) populations observed in the BM of Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice, either 4 or 16 to 20 weeks after poly(I:C) injection; 4 weeks: n = 4 for control, and n = 3 for KO mice; 16 to 20 weeks: n = 5 for control, and n = 4 for KO mice. (B) Percentages of myeloid precursors, common myeloid progenitors (CMPs), granulocyte monocyte progenitors (GMPs), and megakaryocyte erythrocyte progenitors (MEPs), in the BM of Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice after poly(I:C) injection; 4 weeks: n = 4 for control, and n = 2 for KO mice; 16 to 20 weeks: n = 6 for control, and n = 5 for KO mice. (C) Total thymocyte count in ARID1B-deficent or control mice at either 4 or 16 to 20 weeks after poly(I:C)-mediated deletion of Arid1b. (D) Proportion of double-positive (DP), double-negative (DN), CD4+ single-positive (SP), and CD8+ SP populations in Arid1bfl/fl;Mx1-Cre+ and Arid1bfl/fl;Mx1-Cre− mice, either 4 or 16 to 20 weeks after poly(I:C) injection; 4 weeks: n = 7 for control, and n = 5 for KO mice; 16 to 20 weeks: n = 8 for control, and n = 7 for KO mice. (E) Donor chimerism (CD45.2+) in the peripheral blood of recipient mice in competitive repopulation assays. Lethally irradiated recipients received transplantation with either Arid1bfl/fl;Mx1-Cre+ or Arid1bfl/fl;Mx1-Cre− BM mixed in equal proportion with competitor BM (CD45.1+) and deletion of Arid1b was induced 4 weeks after transplantation using poly(I:C). Proportion of donor-derived B cells, T cells, granulocytes, and monocytes were determined every 4 weeks in the peripheral blood of recipient mice (n = 7 for Arid1bfl/fl;Mx1-Cre− and n = 8 for Arid1bfl/fl;Mx1-Cre+). Data are represented as mean ± standard error of the mean (SEM). Statistical difference between WT and KO mice was calculated using unpaired t test and is denoted as ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001.

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