Overlap between a human mesenchymal progenitor subset with murine mesenchymal progenitors whose alteration can promote MDS. (A) Gating strategy for the prospective isolation of mesenchymal subsets in human BM filters. Cells are a mix of BM and digested bone spicules. (B) Frequency of the distinct mesenchymal subsets within the live nonhematopoietic, nonendothelial cells. (C) Frequency of wells testing positive for cell growth of sorted single cells. Wells are scored on a scale from 1 (least confluent) to 3 (most confluent). Data represent 6 independent samples and are presented as mean ± standard deviation (SD). Indicated significance was calculated using 2-way analysis of variance; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. (D) Relative expression of Col2A1 and ACAN in SP and DP cells differentiated into the chondrogenic lineage. (E) Oil and alizarin red staining for adipogenic and osteogenic differentiation of human BM or spicules mesenchymal subsets. (F) Heatmap of DEGs between Ocn-labeled osteoblasts and Osx-labeled osteoprogenitors; more than twofold change; FDR < 0.05. (G) Heatmap of DEGs between human mesenchymal subsets (3 independent samples for each); more than twofold change; FDR < 0.05. (H) Overlap of DEGs between different human mesenchymal subsets with DEGs between Ocn- and Osx-labeled mouse mesenchymal cells. x-axis shows the statistical significance of the overlap shown as P value on a log scale. Dot size represents the number of overlapping genes.