Distinct gene expression signature for mesenchymal subsets in patients with MDS. (A) Gating strategy for sorting distinct mesenchymal subsets from BM of patients undergoing hip replacement therapy or patients with MDS. Red gates indicate populations sorted for sequencing. (B) Frequency of mesenchymal subsets demonstrates no significant differences between patients undergoing hip replacement and patients with MDS. (C) Transcriptome-wide principal component analysis (PCA) of distinct mesenchymal subsets demonstrating the separation between patients undergoing hip replacement surgery and patients with MDS for populations DP, S, DN, and N. (D) Venn diagram demonstrating the overlap in DEGs between patients undergoing hip replacement surgery and those with MDS in the different mesenchymal subsets. (E) Heatmaps of DEGs (more than twofold change; FDR < 0.05) in distinct mesenchymal subsets between patients undergoing hip replacement surgery and patients with MDS. (F) Functional gene categories enriched in DP, DN, and S subsets of patients with MDS. (G) GSEA demonstrating decreased osteogenic differentiation in the S subset of patients with MDS. GO, gene ontology