Accelerated progression of NHD13 MDS in SPP1-KO microenvironment uninjured by irradiation. (A) A mix of NHD13^CD45.2.1:WT^CD45.2 BM cells were transplanted in lethally irradiated CD45.2 WT (n = 5) or Spp1-KO (n = 4) mice at a ratio of 1:1. Mice were tracked via monthly peripheral blood analysis. (B-C) Chimerism analysis of donor cells in WT and SPP1-KO recipients. (D-E) A trend of higher chimerism of NHD13 donor cells in Spp1-KO than in WT recipients. (F) Peripheral blood RBC count, (G) hemoglobin, and (H) MCV. (I) Survival analysis of WT and Spp1-KO animals that received NHD13 transplant. Data in panels F-H are presented as mean ± SD; ∗P < .05; ∗∗P < .01. Statistical analysis was calculated using multiple Student t test.