Figure 1.
Sedimentation coefficient distribution of FVIII-containing immune complexes. FVIII-ICs were formed by incubation of a nearly equimolar ratio of rhFVIII (20 μg/mL or 7.1 × 10−8 mol/L) and AF488-labeled Ab1-7 (10 μg/mL or 6.7 × 10−8 mol/L, compare Table 1). Continuous c(s) distribution analysis of FVIII-IC was obtained from sedimentation velocity area under the curve with fluorescence detection. The free IgG fraction is marked by an arrow. The inset shows a sedimentation profile of labeled antibodies alone. Representative sedimentation coefficient distributions from 2 independent experiments are shown.
