Immune compartment transcriptional analysis in aGVHD of colon. (A) Two clusters visualized in this unsupervised heat map, IC1 and IC2. Rows represent genes, and each column is an AOI. (B-C) A preponderance of AOIs from LG pathology areas and SS patients in IC2. P value calculated by Fisher exact test. ∗∗∗P = .0007; ∗∗∗∗P < .0001. (D) Hallmark gene set enrichment for IC2 genes with interferon gamma (IFN-γ) response. (E-F) Gene Ontology enrichment for the genes upregulated in IC1 shows involvement of multifaceted DNA-binding pathways principally directed toward cell cycle regulation. (G) Volcano plot of differentially expressed genes between IC1 and IC2. FDR, false discovery rate; RNA Pol II transcrpn. regul. seq. sp. DNA binding, RNA polymerase II transcription regulatory region sequence-specific DNA binding.