Figure 2.
CD8 T cells require TCR stimulus in the presence of IL-18 for hyperactivation. (A) Percentage of CD8 T cells producing IFN-γ after reactivation with or without TCR restimulation for 24 hours in the presence (dark green) or absence of 50 ng/mL recombinant murine IL-18. Each point represents an average of 3 wells in an experiment, 9 experiments shown. Analysis by two-way ANOVA. (B) Percentage of CD8 T cells producing IFN-γ after reactivation with or without TCR restimulation for 24 hours in the presence or absence of 50 ng/mL recombinant murine IL-18 and/or 10 ng/mL recombinant murine IL-12. Graph shows representative data from 2 independent experiments; each dot is a technical replicate. P value is based on one-way ANOVA with Tukey post hoc test, comparing cytokine addition within resting and CD3/CD28 groups separately.

CD8 T cells require TCR stimulus in the presence of IL-18 for hyperactivation. (A) Percentage of CD8 T cells producing IFN-γ after reactivation with or without TCR restimulation for 24 hours in the presence (dark green) or absence of 50 ng/mL recombinant murine IL-18. Each point represents an average of 3 wells in an experiment, 9 experiments shown. Analysis by two-way ANOVA. (B) Percentage of CD8 T cells producing IFN-γ after reactivation with or without TCR restimulation for 24 hours in the presence or absence of 50 ng/mL recombinant murine IL-18 and/or 10 ng/mL recombinant murine IL-12. Graph shows representative data from 2 independent experiments; each dot is a technical replicate. P value is based on one-way ANOVA with Tukey post hoc test, comparing cytokine addition within resting and CD3/CD28 groups separately.

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